Analysis of the relative supercooling enhancement of two emerging supercooling techniques

We present herein an experimental study on the ice-nucleation kinetics of two recently introduced aqueous supercooling modalities—oil-sealed isobaric and isochoric supercooling. A series constant-cooling rate experiments compare apparent nucleation temperatures pure water supercooled under these modalities with conventional open-air supercooling, demonstrating that both methods significantly enhance supercoolability system as compared to However, while mean are statistically comparable, displays approximately half variability oil-sealed which may have important implications design supercooling-based biopreservation protocols in stability reproducibility paramount.